Anti-FSH receptor antibody

Anti-FSH receptor antibody

• 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse,Sheep

• 验证应用WB,IHC-P,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human FSH receptor 21-100/695

• 偶联Non-conjugated

• Anti-FSH receptor antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Cell membrane; Multi-pass membrane protein.

• 其它名称Follicle-stimulating hormone receptor precursor
  Follicle stimulating hormone receptor
  Follicle stimulating hormone receptor isoform 1
  Follitropin receptor
  FSHRO
  LGR1
  ODG1
  Follicle-stimulating hormone receptor
  FSH-R
  FSH R
  Fshr
  FSHR_HUMAN
  MGC141667
  MGC141668
  ovarian dysgenesis 1.

  more

• Anti-FSH receptor antibody应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:1ug/Test

• 

  Fig1: Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (FSH receptor ) Polyclonal Antibody, Unconjugated (at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.

  

  Fig2: Sample: Ovary (mouse) Lysate at 40 ug
  Primary: Anti- FSH receptorat 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 78kD
  Observed band size: 78kD
  

  

  Fig3: Blank control (blue line): MCF7 (fixed with 70% methanol overnight at 4℃).
  Primary Antibody (green line): Rabbit Anti-FSH receptor antibody ,Dilution: 0.2μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG .
  Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.

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