Anti-CDKN1A/P21 antibody

Anti-CDKN1A/P21 antibody

• 种属反应性Human,Mouse,Rat,Dog,Rabbit

• 验证应用WB,IHC-P,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human P21 21-100/164

• 偶联Non-conjugated

• Anti-CDKN1A/P21 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Cytoplasmic and Nuclear.

• 其它名称Activating Fragment 1
  CAP20
  Cation chloride cotransporter-interacting protein 1
  CDK Interacting Protein 1
  CDK-interacting protein 1
  CDKI
  CDKN 1
  CDKN1
  CDKN1A
  CIP1
  Cyclin Dependent Kinase Inhibitor 1A
  Cyclin-dependent kinase inhibitor 1
  Cyclin-dependent kinase inhibitor 1A (P21)
  Cyclin-dependent kinase inhibitor 1A (p21, Cip1)
  DNA Synthesis Inhibitor
  MDA 6
  MDA-6
  MDA6
  Melanoma Differentiation Associated Protein 6
  Melanoma differentiation-associated protein 6
  Melanoma differentiation-associated protein
  p21
  P21 protein
  p21CIP1
  p21WAF
  PIC1
  SDI1
  SLC12A9
  WAF1
  Wildtype p53 Activating Fragment 1
  Wildtype p53-activated fragment 1
  CDN1A_HUMAN.

  more

• Anti-CDKN1A/P21 antibody应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:1μg/Test

• 

  Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (P21) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

  

  Fig2: Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-P21 Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
  

  

  Fig3: Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-P21 Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
  

  

  Fig4: Protein: MG-63(human) lysate at 40ug;
  Primary: rabbit Anti-P21 at 1:300;
  Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
  Predicted band size: 18 kD
  Observed band size: 18 kD

  

  Fig5: Tissue/cell: human hepatoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-P21 Polyclonal Antibody, Unconjugate1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
  

  

  Fig6: Blank control(blue): Hep G2 Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
  Primary Antibody: Rabbit Anti-TNFR1/FITC Conjugated antibody Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.

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