Anti-CD86 antibody

Anti-CD86 antibody

• 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Sheep

• 验证应用WB,IHC-P,ICC/IF,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from the middle of human CD86 140-175/313

• 偶联Non-conjugated

• Anti-CD86 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Cell membrane; Single-pass type I membrane protein.

• 其它名称CD28LG2
  FUN1
  LAB72
  Activation B7 2 antigen
  Activation B7-2 antigen 3
  CD86_HUMAN
  Activation B7-2 antigen
  Activation B72 antigen
  B lymphocyte activation antigen B7 2
  B lymphocyte activation antigen B72
  B-lymphocyte activation antigen B7-2 2
  B-lymphocyte activation antigen B7-2
  B7 2
  B7 2 antigen
  B7
  B7-2
  B7.2
  B70
  B72 antigen
  BU63
  CD 86
  CD28 antigen ligand 2 2
  CD28 antigen ligand 2
  Cd28l2
  CD28LG2
  CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2
  CD86 antigen
  CD86 molecule
  CLS1
  CTLA 4 counter receptor B7 2
  CTLA 4 counter receptor B7.2
  CTLA-4 counter-receptor B7.2 2, 3
  CTLA4 counter receptor B72
  Early T-cell costimulatory molecule 1
  ETC-1
  FUN 1
  LAB72
  Ly-58
  Ly58
  MB7
  MB7-2
  Membrane glycoprotein
  MGC34413
  T lymphocyte activation antigen CD86
  T-lymphocyte activation antigen CD86
  TS/A-2.

  more

• Anti-CD86 antibody应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:1μg/Test
  IF:1:100-500
  ICC/IF:1:100-500

• 

  Fig1: Tissue/cell: BV-2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37℃ for 20 min; Antibody incubation with (CD86) Polyclonal Antibody, Unconjugated  1:200, 90 minutes at 37℃; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37℃ for 90 minutes, DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.

  

  Fig2: Sample:
  Lymph node(Mouse)Cell Lysate at 40 ug
  Primary: Anti-CD86 t 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 31 kD
  Observed band size: 31 kD
  

  

  Fig3: Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-CD86/B7-2 Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
  

  

  Fig4: Sample:
  Brain(Rat) lysates at 30ug;
  Heart(Rat) lysates, 30ug;
  Primary: Anti-CD86/B7-2 at 1:200;
  Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000;
  ECL excitated the fluorescence;
  Predicted band size : 34kD
  Observed band size : 34kD
  

  

  Fig5: Blank control: U937(blue).
  Primary Antibody: Rabbit Anti-CD86 antibody, Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.
  Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
  Protocol
  The cells were fixed with 2% paraformaldehyde (10 min).Primary antibody ( 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

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