Anti-CD272 antibody

Anti-CD272 antibody

• 种属反应性Human,Mouse,Rat

• 验证应用WB,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human B and T-lymphocyte attenuator 221-289/289

• 偶联Non-conjugated

• Anti-CD272 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Membrane; Single-pass type I membrane protein (Potential).

• 其它名称B and T lymphocyte associated protein
  B and T lymphocyte attenuator
  B and T lymphocyte associated
  BTLA
  BTLA1
  CD272 antigen
  FLJ16065
  MGC129743
  BTLA_HUMAN
  B- and T-lymphocyte attenuator
  B- and T-lymphocyte-associated protein
  CD272.

  more

• Anti-CD272 antibody应用

  WB:1:500-2000
  FC:1μg /test

• 

  Fig1: Blank control: Jurkat cells(blue).
  Primary Antibody:Rabbit Anti- CD272 antibody Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
  Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
  Protocol
  The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody  were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

  

  Fig2: Sample: Lymph node (Mouse) Lysate at 40 ug
  Primary: Anti-CD272 at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
  Predicted band size: 28 kD
  Observed band size: 48 kD

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