Anti-CD30 antibody

Anti-CD30 antibody

• 种属反应性Human,Pig,Rabbit

• 验证应用WB,IHC-P,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human CD30 131-230/595

• 偶联Non-conjugated

• Anti-CD30 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Isoform Long: Cell membrane; Single-pass type I membrane protein. Isoform Short: Cytoplasm.

• 其它名称CD 30
  CD30
  CD30 antigen
  CD30L receptor
  Cytokine receptor CD30
  D1S166E
  KI 1
  Ki 1 antigen
  Ki-1 antigen
  KI1
  Lymphocyte activation antigen CD30
  TNFRSF 8
  TNFRSF8
  TNFRSF8
  TNR8_HUMAN
  Tumor Necrosis Factor Receptor Superfamily Member 8
  Tumor Necrosis Factor Receptor Superfamily Member 8.

  more

• Anti-CD30 antibody应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:0.2μg/Test

• 

  Fig1: Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CD30) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

  

  Fig2: Blank control(blue): U937 (fixed with 2% paraformaldehyde (10 min)).
  Primary Antibody:Rabbit Anti-CD30 antibody(, Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
  Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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