Anti-CD167b/DDR2 antibody
种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit
验证应用WB,FC
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human CD167b 245-350/855
偶联Non-conjugated
Anti-CD167b/DDR2 antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Cell membrane; Single-pass type I membrane protein.
其它名称DDR 2
DDR2
DDR 2
CD167b antigen
Cell migration inducing protein 20
Discoidin domain containing receptor 2
Discoidin domain receptor 2
Discoidin domain receptor family member 2v Hydroxyaryl protein kinase
MIG20a
Migration inducing gene 16 protein
Neurotrophic tyrosine kinase
Neurotrophic tyrosine kinase receptor related 3v NTRKR 3
NTRKR3v Receptor protein tyrosine kinase TKTv Receptor related 3
TKTv TYRO 10
TYRO10
Tyrosine kinase receptor related to neurotrophic TRK
Tyrosine protein kinase TYRO 10
Tyrosine protein kinase TYRO10
Tyrosylprotein kinase
DDR2_HUMAN
Discoidin domain-containing receptor 2
CD167 antigen-like family member B
Discoidin domain-containing receptor tyrosine kinase 2
Neurotrophic tyrosine kinase, receptor-related 3
Receptor protein-tyrosine kinase TKT
CD167b.
more
Anti-CD167b/DDR2 antibody应用
WB:1:500-2000
FC:1ug/test
Fig1: Sample:
Heart(Mouse) Cell Lysate at 40 ug
Primary: Anti-CD167b/DDR2 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 92 kD
Observed band size: 92 kD
Fig2: Blank control: U87MG.
Primary Antibody (green line): Rabbit Anti-CD167b/DDR2 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Fig3: Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti-DDR2 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 92 kD
Observed band size: 92 kD
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