Fig2: Blank control(Blue): HUVEC cells(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody:Rabbit Anti-CD31 antibody, Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange),used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.