Anti-CD276 antibody
种属反应性Human, Mouse
验证应用WB,ICC,IHC-P,FC
抗体类型兔多抗
免疫原Recombinant protein within human CD276 280-500 aa.
偶联Non-conjugated
Anti-CD276 antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Protein affinity purified.
亚细胞定位Membrane.
其它名称4Ig B7 H3 antibody
4Ig-B7-H3 antibody
AU016588 antibody
B7 H3 antibody
B7 homolog 3 antibody
B7-H3 antibody
B7H3 antibody
B7RP-2 antibody
CD_antigen=CD276 antibody
CD276 antibody
CD276 antigen antibody
CD276 molecule antibody
CD276_HUMAN antibody
Costimulatory molecule antibody
Flags: Precursor antibody
PSEC0249 antibody
UNQ309/PRO352 antibody
more
Anti-CD276 antibody应用
WB: 1:500-1:2,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
Fig1: Western blot analysis of CD276 on 293 and 293T cell lysates using anti-CD276 antibody at 1/500 dilution.
Fig2: ICC staining CD276 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining CD276 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining CD276 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-CD276 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-CD276 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-CD276 antibody. Counter stained with hematoxylin.
Fig8: Flow cytometric analysis of LOVO cells with CD276 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary
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