Anti-DDIT3 antibody
种属反应性Human,Mouse
验证应用WB,FC
抗体类型兔多抗
免疫原Synthetic peptide within human DDIT3 aa 70-110.
偶联Non-conjugated
Anti-DDIT3 antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Peptide affinity purified.
亚细胞定位Nucleus.
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Anti-DDIT3 antibody应用
WB: 1:500-1:2,000
FC: 1:50-1:100
Fig1: Western blot analysis of DDIT3 on different lysates using anti-DDIT3 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse testis tissue
Lane 2: LOVO
Lane 3: Mouse brain tissue
Fig2: Flow cytometric analysis of HepG2 cells with DDIT3 antibody at 1/50 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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