Anti-Orai3 antibody

Anti-Orai3 antibody

• 种属反应性Human,Mouse,Rat

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型兔多抗

• 免疫原Synthetic peptide within human Orai-3 aa 10-50.

• 偶联Non-conjugated

• Anti-Orai3 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified.

• 亚细胞定位Membrane. Multi-pass membrane protein.

• 其它名称MGC13024 antibody
  Orai 3 antibody
  ORAI calcium release activated calcium modulator 3 antibody
  Orai3 antibody
  ORAI3_HUMAN antibody
  Protein orai 3 antibody
  Protein orai-3 antibody
  TMEM 142C antibody
  TMEM142C antibody
  Transmembrane protein 142C antibody

  more

• Anti-Orai3 antibody应用

  WB: 1:500-1:1,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

• 

  Fig1: Western blot analysis of Orai3 using anti-Orai3 antibody at 1/500 dilution.
  Lane 1 : Raji cell lysates
  Lane 2 : Raji cell lysates(anti-Orai3 antibody+ immunizing peptide)
  

  

  Fig2: ICC staining Orai3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig3: ICC staining Orai3 in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig4: ICC staining Orai3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Orai3 antibody. Counter stained with hematoxylin.

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Orai3 antibody. Counter stained with hematoxylin.

  

  Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Orai3 antibody. Counter stained with hematoxylin.

  

  Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Orai3 antibody. Counter stained with hematoxylin.

  

  Fig9: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Orai3 antibody. Counter stained with hematoxylin.

  

  Fig10: Flow cytometric analysis of A549 cells with Orai3 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the sec

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