Anti-USP13 antibody

Anti-USP13 antibody

• 种属反应性Human,Mouse,Rat

• 验证应用WB,ICC,FC,IHC-P

• 抗体类型兔多抗

• 免疫原Recombinant protein within human USP13 aa 550-800.

• 偶联Non-conjugated

• Anti-USP13 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Protein affinity purified.

• 亚细胞定位Nucleus. Cytosol.

• 其它名称Deubiquitinating enzyme 13 antibody
  Isopeptidase T 3 antibody
  Isopeptidase T-3 antibody
  Isopeptidase T3 antibody
  ISOT 3 antibody
  ISOT-3 antibody
  ISOT3 antibody
  Ubiquitin carboxyl terminal hydrolase 13 antibody
  Ubiquitin carboxyl-terminal hydrolase 13 antibody
  Ubiquitin specific peptidase 13 (isopeptidase T3) antibody
  Ubiquitin specific peptidase 13 antibody
  Ubiquitin specific processing protease 13 antibody
  Ubiquitin specific protease 13 antibody
  Ubiquitin thioesterase 13 antibody
  Ubiquitin thiolesterase 13 antibody
  Ubiquitin-specific-processing protease 13 antibody
  UBP13 antibody
  UBP13_HUMAN antibody
  USP 13 antibody
  USP13 antibody

  more

• Anti-USP13 antibody应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  FC: 1:50-1:100
  IHC-P: 1:100-1:200

• 

  Fig1: Western blot analysis of USP13 on different lysates using anti-USP13 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: 293T
  Lane 2: Daudi
  Lane 3: Mouse testis
  Lane 4: Rat brain
  

  

  Fig2: ICC staining USP13 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig3: ICC staining USP13 in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig4: Flow cytometric analysis of A431 cells with USP13 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

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