Anti-Arginase II antibody
种属反应性Human,Mouse,Rat,Chicken,Dog,Cow,Rabbit
验证应用WB,IHC-P,ICC/IF
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human Arginase II 181-290/354
偶联Non-conjugated
Anti-Arginase II antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Cytoplasm.
其它名称ARG 2
ARG2
ARGI2_HUMAN
Arginase-2
Arginase 2Arginase2
Arginase liver
Arginase type II
Arginase II
ArginaseII
Arginase-II
Arginase1
Arginase-2, mitochondrial
Kidney-type arginase
Non-hepatic arginase
Type II arginase
.
more
Anti-Arginase II antibody应用
WB:1:500-2000
IHC-P:1:400-800
IF:1:100-500
ICC/IF:1:100-500
Fig1: Sample:
Small intestine (Mouse) Lysate at 40 ug
Primary: Anti-Arginase II (Bs- 11397R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 36 kD
Fig2: Sample:
DU145(human)cell Lysate at 30 ug
Primary: Anti-Arginase II at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36kD
Observed band size: 36 kD
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Arginase II) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig4: Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Arginase II) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig5: Sample: Small intestine (Mouse) Lysate at 40 ug
Primary: Anti-Arginase II at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 36 kD
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