Anti-Bcl2 antibody
种属反应性Human,Mouse
验证应用WB,ICC,FC
抗体类型兔多抗
免疫原Synthetic peptide within human BCL-2 20-100 aa.
偶联Non-conjugated
Anti-Bcl2 antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Peptide affinity purified.
亚细胞定位Mitochondrion. Endoplasmic reticulum. Nucleus.
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Anti-Bcl2 antibody应用
WB: 1:200-1:500
ICC: 1:50-1:200
FC: 1:50-1:100
Fig1: Western blot analysis of Bcl2 on different lysates using anti-Bcl2 antibody at 1/200 dilution.
Positive control:
Lane 1: HL-60
Lane 2: Mouse colon
Fig2: ICC staining Bcl2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Bcl2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Bcl2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Flow cytometric analysis of MCF-7 cells with Bcl2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
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