Anti-B7-H4(VTCN1) antibody
种属反应性Human,Rat
验证应用WB,ICC,IHC-P,FC
抗体类型兔多抗
免疫原Recombinant protein with human B7-H4 aa 50-220.
偶联Non-conjugated
Anti-B7-H4(VTCN1) antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Protein affinity purified.
亚细胞定位Cell membrane.
其它名称B7 family member, H4 antibody
B7 H4 antibody
B7 homolog 4 antibody
B7 superfamily member 1 antibody
B7 superfamily, member 1 antibody
B7-H4 antibody
B7h.5 antibody
B7h4 antibody
B7S1 antibody
B7x antibody
BC032925 antibody
Immune costimulatory protein B7-H4 antibody
Immune costimulatory protein B7H4 antibody
MGC41287 antibody
PRO1291 antibody
Protein B7S1 antibody
RP11 229A19.4 antibody
T cell costimulatory molecule B7x antibody
T-cell costimulatory molecule B7x antibody
V set domain-containing T cell activation inhibitor 1 antibody
V-set domain-containing T-cell activation inhibitor 1 antibody
VCTN1 antibody
Vtcn1 antibody
VTCN1_HUMAN antibody
more
Anti-B7-H4(VTCN1) antibody应用
WB: 1:500-1:2000
ICC: 1:1,000
IHC-P: 1:50-1:200
FC: 1:50-1:100
Fig1: Western blot analysis of B7-H4(VTCN1) on SKBR3 cell and human colon tissue lysates using anti-B7-H4(VTCN1) antibody at 1/500 dilution.
Fig2: ICC staining B7-H4(VTCN1) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-B7-H4(VTCN1) antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-B7-H4(VTCN1) antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-B7-H4(VTCN1) antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of MCF-7 cells with B7-H4(VTCN1) antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
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