Anti-APE1 antibody

Anti-APE1 antibody

• 种属反应性Human,Mouse,Rat

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型兔多抗

• 免疫原Recombinant protein with Human APE1 aa 1-250.

• 偶联Non-conjugated

• Anti-APE1 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Protein affinity purified.

• 亚细胞定位Nucleus. Endoplasmic reticulum.

• 其它名称AP endonuclease 1 antibody
  AP endonuclease class I antibody
  AP lyase antibody
  APE 1 antibody
  APE antibody
  APE-1 antibody
  APEN antibody
  APEX 1 antibody
  APEX antibody
  APEX nuclease (multifunctional DNA repair enzyme) 1 antibody
  Apex nuclease 1 antibody
  APEX nuclease antibody
  APEX1 antibody
  APEX1_HUMAN antibody
  Apurinic endonuclease antibody
  Apurinic-apyrimidinic endonuclease 1 antibody
  Apurinic/apyrimidinic (abasic) endonuclease antibody
  Apurinic/apyrimidinic endonuclease 1 antibody
  Apurinic/apyrimidinic exonuclease antibody
  APX antibody
  BAP1 antibody
  Deoxyribonuclease (apurinic or apyrimidinic) antibody
  DNA (apurinic or apyrimidinic site) lyase antibody
  DNA-(apurinic or apyrimidinic site) lyase, mitochondrial antibody
  EC 4.2.99.18 antibody
  HAP 1 antibody
  HAP1 antibody
  Human Apurinic endonuclease 1 antibody
  MGC139790 antibody
  Multifunctional DNA repair enzyme antibody
  Redox factor 1 antibody
  Redox factor-1 antibody
  REF 1 antibody
  REF 1 protein antibody
  REF-1 antibody
  REF1 antibody
  REF1 protein antibody

  more

• Anti-APE1 antibody应用

  WB: 1:500-1:2,000
  ICC: 1:500-1:1,000
  IHC-P: 1:100-1:500
  FC: 1:50-1:100

• 

  Fig1: Western blot analysis of APE1 on different lysates using anti-APE1 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: HL-60
  Lane 2: Human skin tissue
  Lane 3: MCF-7
  Lane 4: Mouse placenta tissue
  

  

  Fig2: ICC staining APE1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig3: ICC staining APE1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig4: ICC staining APE1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-APE1 antibody. Counter stained with hematoxylin.

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-APE1 antibody. Counter stained with hematoxylin.

  

  Fig7: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-APE1 antibody. Counter stained with hematoxylin.

  

  Fig8: Flow cytometric analysis of A549 cells with APE1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary

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