Anti-BubR1 antibody
种属反应性Human
验证应用WB,ICC,IHC-P,FC
抗体类型兔多抗
免疫原Recombinant protein within human BubR1 330-600 aa.
偶联Non-conjugated
Anti-BubR1 antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Protein affinity purified.
亚细胞定位Nucleus. Cytoplasm.
数据链接O60566 Human
其它名称Beta homolg of S. cerevisiae BUB 1 antibody
Beta homolg of S. cerevisiae budding uninhibited by benzimidazoles antibody
BUB 1B antibody
BUB1 budding uninhibited by benzimidazoles 1 homolog beta antibody
Bub1A antibody
BUB1B antibody
BUB1B_HUMAN antibody
BUB1beta antibody
BUBR1 antibody
Budding Uninhibited by Benzimidazoles 1 beta antibody
Budding uninhibited by benzimidazoles 1 homolog beta (yeast) antibody
hBUBR1 antibody
MAD3/BUB1 related protein kinase antibody
MAD3/BUB1-related protein kinase antibody
MAD3L antibody
Mitotic checkpoint gene BUB1B antibody
Mitotic checkpoint kinase MAD3L antibody
Mitotic checkpoint serine/threonine protein kinase BUB1 beta antibody
Mitotic checkpoint serine/threonine-protein kinase BUB1 beta antibody
MVA1 antibody
OTTHUMP00000160319 antibody
Protein SSK1 antibody
SSK 1 antibody
SSK1 antibody
more
Anti-BubR1 antibody应用
WB: 1:5,000-1:10,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
Fig1: Western blot analysis of BubR1 on SH-SY-5Y cell lysate using anti-BubR1 antibody at 1/10,000 dilution.
Fig2: ICC staining BubR1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining BubR1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining BubR1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-BubR1 antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of HL-60 cells with BubR1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
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