Anti-Carbonic anhydrase 2 antibody
种属反应性Human
验证应用WB,ICC,IHC-P
抗体类型兔多抗
免疫原Recombinant protein with human Carbonic anhydrase 2 aa 50-220.
偶联Non-conjugated
Anti-Carbonic anhydrase 2 antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Protein affinity purified.
亚细胞定位Cytoplasm. Cell membrane.
其它名称can antibody
CAN_ECOLI antibody
Carbonate dehydratase 2 antibody
Carbonic anhydrase 2 antibody
Anti-Carbonic anhydrase 2 antibody应用
WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
Fig1: Western blot analysis of Carbonic anhydrase 2 on different cell lysates using anti-Carbonic anhydrase 2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: 293
Lane 2: THP-1
Lane 3: HL-60
Fig2: ICC staining Carbonic anhydrase 2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Carbonic anhydrase 2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Carbonic anhydrase 2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Carbonic anhydrase 2 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Carbonic anhydrase 2 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-Carbonic anhydrase 2 antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Carbonic anhydrase 2 antibody. Counter stained with hematoxylin.
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