Anti-Dnmt3b antibody
种属反应性Human,Mouse,Rat
验证应用WB,ICC,IHC-P,FC
抗体类型兔多抗
免疫原Synthetic peptide within human Dnmt3b aa 1-50.
偶联Non-conjugated
Anti-Dnmt3b antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Peptide affinity purified.
亚细胞定位Nucleus.
其它名称Cytosine 5methyltransferase 3B antibody
DNA antibody
DNA (cytosine 5) methyltransferase 3 beta antibody
DNA (cytosine 5)-methyltransferase 3B antibody
DNA (cytosine-5)-methyltransferase 3B antibody
DNA methyltransferase HsaIIIB antibody
DNA MTase HsaIIIB antibody
DNM3B_HUMAN antibody
Dnmt3b antibody
EC 2.1.1.37 antibody
ICF antibody
ICF1 antibody
M.HsaIIIB antibody
MGC124407 antibody
RP23-89H14.3 antibody
more
Anti-Dnmt3b antibody应用
WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
Fig1: Western blot analysis of Dnmt3b on K562 cell lysate using anti-Dnmt3b antibody at 1/500 dilution.
Fig2: ICC staining Dnmt3b in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Dnmt3b antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Dnmt3b antibody. Counter stained with hematoxylin.
Fig5: Flow cytometric analysis of K562 cells with Dnmt3b antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
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