Anti-Cytokeratin 8 antibody
种属反应性Human,Mouse,Rat
验证应用WB,ICC,IHC-P,FC
抗体类型兔多抗
免疫原Peptide
偶联Non-conjugated
Anti-Cytokeratin 8 antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Peptide affinity purified
亚细胞定位Cytoplasm. Nucleus.
其它名称CARD2 antibody
CK 8 antibody
CK-8 antibody
CK8 antibody
CYK8 antibody
CYKER antibody
Cytokeratin endo A antibody
Cytokeratin-8 antibody
DreK8 antibody
EndoA antibody
K2C8 antibody
K2C8_HUMAN antibody
K8 antibody
Keratin 8 antibody
Keratin type II cytoskeletal 8 antibody
Keratin, type II cytoskeletal 8 antibody
Keratin-8 antibody
KO antibody
Krt 2.8 antibody
KRT8 antibody
MGC118110 antibody
MGC174782 antibody
MGC53564 antibody
MGC85764 antibody
sb:cb186 antibody
Type-II keratin Kb8 antibody
more
Anti-Cytokeratin 8 antibody应用
WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
Fig1: Western blot analysis of Cytokeratin 8 on different lysates using anti-Cytokeratin 8 antibody at 1/500 dilution.
Positive control:
Lane 1: Human small intestine
Lane 2: A431
Fig2: ICC staining Cytokeratin 8 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Cytokeratin 8 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Cytokeratin 8 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin.
Fig9: Flow cytometric analysis of MCF-7 cells with Cytokeratin 8 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the
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