Anti-Aquaporin 4 antibody

Anti-Aquaporin 4 antibody

• 种属反应性Human,Mouse,Rat,Pig,Cow,Rabbit,Sheep

• 验证应用WB,IHC-P,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human AQP4 271-323/323

• 偶联Non-conjugated

• Anti-Aquaporin 4 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Membrane; Multi-pass membrane protein.

• 其它名称aquaporin 4
  WCH4
  Mercurial-insensitive water channel
  MIWC
  AQP 4
  AQP4
  Aquaporin4
  HMIWC 2
  HMIWC2
  Mercurial insensitive water channel
  MGC22454
  MIWC
  AQP4_HUMAN
  Aquaporin-4
  AQP-4.

  more

• Anti-Aquaporin 4 antibody应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:1μg /test

• 

  Fig1: Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-AQP4 Polyclonal Antibody, Unconjugated1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
  

  

  Fig2: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-AQP4 Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
  

  

  Fig3: Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-AQP4 Polyclonal Antibody, Unconjugated:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
  

  

  Fig4: Sample: mouse lung lysate at 30ug;
  Primary: Anti-Aquaporin 4  at 1:300;
  Secondary: HRP conjugated Goat-Anti-rabbit IgG(bse-0295G-HRP) at 1: 5000;
  Predicted band size:36 kD
  Observed band size:34 kD

  

  Fig5: Sample: Muscle(Mouse) lysate at 30ug; Primary: Anti-Aquaporin 4  at 1:300; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size:36kD Observed band size:35kD

  

  Fig6: Blank control: K562 (blue).
  Primary Antibody:Rabbit Anti-Aquaporin 4 antibody  Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions;
  Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
  Protocol
  The cells were fixed with 2% paraformaldehyde for 10 min at 37℃. Primary antibody were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min at room temperature. Acquisition of 20,000 events was performed.

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