Anti-Angiotensin II type 1A receptor antibody
种属反应性Human,Mouse,Rat
验证应用WB,IHC-P,FC
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from the middle of human Angiotensin II type 1A receptor 101-200/359
偶联Non-conjugated
Anti-Angiotensin II type 1A receptor antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式Affinity purified by Protein A
亚细胞定位Cell membrane; Multi-pass membrane protein.
其它名称AGTR1
Agtr1a
AT1
AT1A
AT1AR
Type 1 angiotensin II receptor
AGTR1_HUMAN
AGTR1B
AT2R1
AT2R1B.
more
Anti-Angiotensin II type 1A receptor antibody应用
WB:1:500-2000
IHC-P:1:400-800
FC:3ug/Test
Fig1: Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti- Angiotensin II type 1A receptor at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 42 kD
Fig2: Blank control: U87MG.
Primary Antibody (green line): Rabbit Anti-Angiotensin II type 1A receptor antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Fig3: Sample:
Liver (Mouse) Lysate at 40 ug
Lung (Mouse) Lysate at 40 ug
Adrenal gland (Rat) Lysate at 40 ug
Primary: Anti-Angiotensin II type 1A receptor at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 56 kD
Fig4: Sample:
A549(Human) Cell Lysate at 430 ug
Primary: Anti-Angiotensin II type 1A receptorat 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41/51 kD
Fig5: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Angiotensin II type 1A receptor) Polyclonal Antibody, Unconjugatedt 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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