Anti-Alpha-ENaC antibody

Anti-Alpha-ENaC antibody

• 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse,Sheep

• 验证应用WB,IHC-P,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human Alpha-ENaC 201-300/669

• 偶联Non-conjugated

• Anti-Alpha-ENaC antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Apical cell membrane; Multi-pass membrane protein.

• 其它名称Alpha ENaC 2
  Alpha ENaC
  Alpha NaCH
  Alpha-ENaC
  Alpha-NaCH
  Amiloride sensitive epithelial sodium channel alpha subunit
  Amiloride sensitive sodium channel subunit alpha
  Amiloride-sensitive sodium channel subunit alpha
  ENaCa
  ENaCalpha
  Epithelial Na(+) channel subunit alpha
  Epithelial Na+ channel subunit alpha
  FLJ21883
  Nonvoltage gated sodium channel 1 subunit alpha
  Nonvoltage-gated sodium channel 1 subunit alpha
  SCNEA
  SCNN 1
  SCNN1
  SCNN1A
  SCNNA_HUMAN
  Sodium channel nonvoltage gated 1 alpha.

  more

• Anti-Alpha-ENaC antibody应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:5ug/Test

• 

  Fig1: Sample:
  Kidney (Mouse) Lysate at 40 ug
  Primary: Anti-Alpha-ENaC at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 76 kD
  Observed band size: 77 kD
  

  

  Fig2: Sample:
  Kidney (Rat) Lysate at 40 ug
  Primary: Anti-Alpha-ENaC at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 76 kD
  Observed band size: 77 kD
  

  

  Fig3: Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-Alpha-ENaC Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibodyand DAB(C-0010) staining
  

  

  Fig4: Blank control: A549(blue), the cells were fixed with 2% paraformaldehyde (10 min) and .
  Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 5μg in 100 μL1X PBS containing 0.5% BSA(green).

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