Anti-Phospho-P38 MAPK (Thr180 + Tyr182) antibody

Anti-Phospho-P38 MAPK (Thr180 + Tyr182) antibody

• 种属反应性Human,Mouse,Rat

• 验证应用WB,IHC-P,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated Synthesised phosphopeptide derived from human p38 MAPK around the phosphorylation site of Thr180/Tyr182: M(p-T)G(p-Y)VA.

• 偶联Non-conjugated

• Anti-Phospho-P38 MAPK (Thr180 + Tyr182) antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃. Avoid repeated freeze/thaw cycles.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式ProA affinity purified.

• 亚细胞定位Cytoplasm. Nucleus.

• Anti-Phospho-P38 MAPK (Thr180 + Tyr182) antibody应用

  WB: 1:500-1:2,000
  IHC-P: 1:400-1:800
  FC: 1:50

• 

  Fig1: Western blot analysis of Phospho-P38 MAPK (Thr180 + Tyr182) on different tissue lysates using anti-Phospho-P38 MAPK (Thr180 + Tyr182) at 1/300 dilution.
  Positive control:
  Line 1: Mouse muscle tissue
  Line 2: Rat muscle tissue

  

  Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Phospho-P38 MAPK (Thr180 + Tyr182) antibody. Counter stained with hematoxylin.

  

  Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-P38 MAPK (Thr180 + Tyr182) antibody. Counter stained with hematoxylin.

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Phospho-P38 MAPK (Thr180 + Tyr182) antibody. Counter stained with hematoxylin.

  

  Fig5: Flow cytometric analysis of HepG2 cells with Phospho-P38 MAPK (Thr180 + Tyr182) antibody at 1/50 dilution (green) compared with blank control (blue) and isotype control (orange). Goat anti-rabbit IgG-FITC was used as the secondary antibody (white blue).

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