Anti-5HT4 Receptor antibody

Anti-5HT4 Receptor antibody

• 种属反应性Human,Mouse,Rat,Chicken,Cow

• 验证应用WB,IHC-P,ICC/IF

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human 5-HTR4 21-120/388

• 偶联Non-conjugated

• Anti-5HT4 Receptor antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Cell membrane; Multi-pass membrane protein. Endosome. Note=Interaction with SNX27 mediates recruitment to early endosomes, while interaction with SLC9A3R1 and EZR might target the protein to specialized subcellular regions, such as microvilli.

• 其它名称5-HTR4 (5-hydroxytryptamine (serotonin) receptor 4a)
  Htr4a
  5-HT4
  5-HT4A
  5HT4
  Htr45 HT 4
  5 HT4
  5 HT4R
  5 hydroxytryptamine 4 receptor
  5 hydroxytryptamine receptor 4
  5 hydroxytryptamine serotonin receptor 4
  5 hydroxytryptamine4 receptor
  5HT4
  5HT4R
  Cardiac 5 HT4 receptor
  HTR 4
  HTR4
  Serotonin 5 HT4 receptor
  Serotonin receptor 4
  5HT4R_HUMAN.

  more

• Anti-5HT4 Receptor antibody应用

  WB:1:500-2000
  IHC-P:1:400-800
  IF:1:200-800

• 

  Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (5HT4 Receptor) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

  

  Fig2: Sample:
  Bone (Mouse) Lysate at 40 ug
  Primary: Anti-5HT4 Receptorat 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 43 kD
  Observed band size: 43 kD
  

  

  Fig3: Sample:
  Large intestine (Mouse) Lysate at 40 ug
  Primary: Anti-5HT4 Receptor at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 43 kD
  Observed band size: 43 kD
  

  

  Fig4: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (5HT4 Receptor) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit)  instructionsand DAB staining.

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