Anti-5HT3B receptor antibody
种属反应性Human,Mouse,Rat,Cow,Rabbit
验证应用WB,IHC-P,FC
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human 5HTR3B/5HT3B receptor 101-200/441
偶联Non-conjugated
Anti-5HT3B receptor antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Cell membrane; Multi-pass membrane protein. Note=Presumably retained within the endoplasmic reticulum unless complexed with HTR3A.
其它名称5 HT3 B
5 HT3B
5 HTR3B
5 hydroxytryptamine 3 receptor B subunit
5 hydroxytryptamine receptor 3B
5 hydroxytryptamine serotonin receptor 3B
5-HT3-B
5-HT3B
5-HTR3B
5-hydroxytryptamine 3 receptor B subunit
5-hydroxytryptamine receptor 3B
5-hydroxytryptamine serotonin receptor 3B
5HT3B_HUMAN
5HTR3B
HTR3B
Serotonin gated ion channel subunit
serotonin receptor 3B.
more
Anti-5HT3B receptor antibody应用
WB:1:500-2000
IHC-P:1:400-800
FC:1ug/Test
Fig1: Blank control(blue):293T Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
Primary Antibody: Rabbit Anti-5HT3B receptor Phospho-c-Fos (Thr325)/AF488 Conjugated antibody , Dilution: 1ug in 100 ul 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (5HT3B receptor) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig3: Sample: 293T Cell (Human) Lysate at 40 ug
Primary: Anti- 5HT3B receptor at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 55 kD
Fig4: Sample: Lovo Cell (Human) Lysate at 40 ug
Primary: Anti- 5HT3B receptorat 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 55 kD
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