Anti-Adenosine A3 Receptor antibody

Anti-Adenosine A3 Receptor antibody

• 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep

• 验证应用WB,IHC-P,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human ADORA3 85-180/347

• 偶联Non-conjugated

• Anti-Adenosine A3 Receptor antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Cell membrane.

• 其它名称adenosine A3 receptor
  A3AR
  AD 026
  AD026
  ADORA 3
  ADORA3
  bA552M11.5
  RP11 552M11.7
  TGPCR1
  adenosine receptor A3 isoform 1
  adenosine receptor A3 isoform 2
  AA3R_HUMAN.

  more

• Anti-Adenosine A3 Receptor antibody应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:1μg /test

• 

  Fig1: Blank control (blue line): Raji (blue)(The cells were fixed with 70% ethanol overnight at -20℃).
  Primary Antibody (green line): Rabbit Anti-Adenosine A3 Receptor antibody; Dilution: 1μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG .
  Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE;Dilution: 1μg /test.
  

  

  Fig2: Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-ADORA3 Polyclonal Antibody, Unconjugated1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
  

  

  Fig3: Sample:
  Colon carcinoma lysates (Human) Lysate at 40 ug
  Gastric carcinoma lysates (Human) Lysate at 40 ug
  Primary: Anti- ADORA3  at 1/300 dilution
  Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
  Predicted band size: 35 kD
  Observed band size: 35 kD
  

  

  Fig4: Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Adenosine A3 Receptor) Polyclonal Antibody, Unconjugated ( at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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