Anti-ADAM23 antibody

Anti-ADAM23 antibody

• 种属反应性Human,Mouse,Rat,Pig,Cow,Horse,Rabbit

• 验证应用WB,IHC-P,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human ADAM23 551-650/832

• 偶联Non-conjugated

• Anti-ADAM23 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Cell membrane; Single-pass type I membrane protein. Isoform Gamma: Secreted.

• 其它名称A disintegrin and metalloproteinase domain 23
  MDC 3
  Metalloproteinase like disintegrin like and cysteine rich protein 3.

• Anti-ADAM23 antibody应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:0.2ug/Test

• 

  Fig1: Blank control: U87MG.
  Primary Antibody (green line): Rabbit Anti-ADAM23 antibody
  Dilution: 1μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG .
  Secondary Antibody : Goat anti-rabbit IgG-PE
  Dilution: 1μg /test.
  The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

  

  Fig2: Sample: Raji Cell (Human) Lysate at 40 ug
  Primary: Anti-ADAM23 at 1/300 dilution
  Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
  Predicted band size: 75-95kD
  Observed band size: 75/35 kD
  

  

  Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ADAM23) Polyclonal Antibody, Unconjugated  at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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