Anti-KCNK18 antibody

Anti-KCNK18 antibody

• 种属反应性Human,Mouse,Rat

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型兔多抗

• 免疫原Synthetic peptide within mouse KCNK18 aa 1-150.

• 偶联Non-conjugated

• Anti-KCNK18 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified.

• 亚细胞定位Cell membrane.

• 其它名称K2p18.1 antibody
  KCNK18 antibody
  KCNKI_HUMAN antibody
  MGR13 antibody
  OTTHUMP00000020575 antibody
  Potassium channel subfamily K member 18 antibody
  TRESK 2 antibody
  TRESK antibody
  TRESK2 antibody
  TRIK antibody
  TWIK related individual K+ channel antibody
  TWIK related individual potassium channel antibody
  TWIK related spinal cord K+ channel antibody
  TWIK related spinal cord potassium channel antibody
  TWIK-related individual potassium channel antibody
  TWIK-related spinal cord potassium channel antibody

  more

• Anti-KCNK18 antibody应用

  WB:1:500-1:2,000
  ICC:1:50-1:100
  IHC-P:1:100-1:500
  FC:1:50-1:100

• 

  Fig1: Western blot analysis of KCNK18 on rat spinal cord tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody  was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  

  Fig2: ICC staining of KCNK18 in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig3: ICC staining of KCNK18 in SHG-44 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig4: Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue using anti-KCNK18 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig5: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-KCNK18 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibod for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-KCNK18 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig7: Flow cytometric analysis of KCNK18 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibod(red). After incubation of the primary antibody at room temperature for an hour, the cells were

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！