Anti-Dopamine Transporter antibody

Anti-Dopamine Transporter antibody

• 种属反应性Human,Mouse,Rat,Dog,Cow

• 验证应用WB,IHC-P,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human DAT1 251-350/620

• 偶联Non-conjugated

• Anti-Dopamine Transporter antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式Affinity purified by Protein A

• 亚细胞定位Membrane; Multi-pass membrane protein.

• 其它名称DA transporter
  DAT 1
  DAT
  DAT1
  Dopamine transporter
  SC6A3_HUMAN
  SLC6A3
  Sodium dependent dopamine transporter
  Sodium-dependent dopamine transporter
  Solute carrier family 6 (neurotransmitter transporter dopamine), member 3
  Solute carrier family 6 member 3
  Variable number tandem repeat (VNTR)
  dopamine transporter
  ADAT 1
  Adenosine deaminase tRNA specific 1
  HADAT1
  tRNA specific adenosine deaminase 1.

  more

• Anti-Dopamine Transporter antibody应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:0.2μg /test

• 

  Fig1: Positive control: RSC96
  Isotype Control Antibody: Rabbit IgG; Secondary Antibody: Goat anti-rabbit IgG-FITC; Dilution: 1:200 in 1 X PBS containing 0.5% BSA
  Primary Antibody catalog number: Dilution: 12μg in 100 μl 1X PBS containing 0.5% BSA

  

  Fig2: Positive control: RSC96
  Isotype Control Antibody: rabbit IgG-PE/CY5, Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 6μg in 100 μL1X PBS containing 0.5% BSA.

  

  Fig3: Blank control: U937 (blue).
  Primary Antibody:Rabbit Anti-Dopamine Transporter antibody , Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol
  The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody  0.2μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

  

  Fig4: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Dopamine Transporter) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

  

  Fig5: Sample:
  Cerebrum (Mouse) Lysate at 40 ug
  Primary: Anti-Dopamine Transporter ( at 1/500 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 68 kD
  Observed band size: 68 kD
  

  

  Fig6: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Dopamine Transporter) Polyclonal Antibody, Unconjugate at 1:400 overnight at 4℃, followed by operating according to SP Kit (Rabbit) (sp-0023) instructionsand DAB staining.

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