Anti-Dopamine beta Hydroxylase antibody

Anti-Dopamine beta Hydroxylase antibody

• 种属反应性Human,Mouse,Rat

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human DBH 471-550/621

• 偶联Non-conjugated

• Anti-Dopamine beta Hydroxylase antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle, secretory vesicle lumen (By similarity). Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen (By similarity). Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type II membra

• 其它名称DBH
  DBM
  Dopamine beta hydroxylase
  Dopamine-Beta-Hydroxylase
  Dopamine beta monooxygenase
  DOPO_RAT
  DOPO_HUMAN.

  more

• Anti-Dopamine beta Hydroxylase antibody应用

  WB:1:500-2000
  IHC-P:1:400-800

• 

  Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (DOPO) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit)  instructionsand DAB staining.

  

  Fig2: Sample:
  Jurkat (Human) Cell Lysate at 40 ug
  Primary: Anti-Dopamine beta Hydroxylase  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 70 kD
  Observed band size: 70 kD

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