Anti-DNCLI1 antibody

Anti-DNCLI1 antibody

• 种属反应性Human,Mouse,Rat,Chicken

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human DNCLI1 451-550/523

• 偶联Non-conjugated

• Anti-DNCLI1 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 其它名称Cytoplasmic dynein 1 light intermediate chain 1
  cytosolic
  DC1L1_HUMAN
  DLC-A
  DNCLI1
  dync1li1
  Dynein cytoplasmic 1 light intermediate chain 1
  Dynein cytoplasmic light intermediate polypeptide 1
  Dynein light chain A
  Dynein light intermediate chain 1
  Dynein light intermediate chain 1 cytosolic
  FLJ10219
  LIC1.

  more

• Anti-DNCLI1 antibody应用

  WB:1:500-2000
  IHC-P:1:400-800

• 

  Fig1: Tissue/cell: Human cervical cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-DNCLI1 Polyclonal Antibody, Unconjugated(ER1907-65) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody and DAB(C-0010) staining
  

  

  Fig2: Sample:
  Heart (Mouse) Lysate at 40 ug
  Primary: Anti- DNCLI1at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 57 kD
  Observed band size: 57 kD

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