Anti-CYP11A1 antibody

Anti-CYP11A1 antibody

• 种属反应性Human,Mouse,Rat

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human CYP11A1/P450SCC 321-420/521

• 偶联Non-conjugated

• Anti-CYP11A1 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式Affinity purified by Protein A

• 亚细胞定位Mitochondrion membrane.

• 其它名称Cholesterol 20 22 desmolase; Cholesterol desmolase; Cholesterol monooxygenase (side chain cleaving); Cholesterol side chain cleavage enzyme; Cholesterol side chain cleavage enzyme mitochondrial; Cholesterol side-chain cleavage enzyme; CP11A_HUMAN; CYP11A; CYP11A1; CYPXIA1; Cytochrome P450 11A1; Cytochrome P450 11A1 mitochondrial; Cytochrome P450 family 11 subfamily A polypeptide 1; Cytochrome P450 subfamily XIA; Cytochrome P450(scc); Cytochrome P450C11A1; mitochondrial; P450SCC; Steroid 20 22 lyase.

• Anti-CYP11A1 antibody应用

  WB:1:500-2000
  IHC-P:1:400-800

• 

  Fig1: Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CYP11A1) Polyclonal Antibody, Unconjugated ( at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

  

  Fig2: Sample:
  Lane 1: Brain(Rat) Lysate at 30 ug
  Lane 2: Kidney(Rat)Lysate at 30 ug
  Primary: Anti-CYP11A1/P450SCC  at 1:200 dilution;
  Secondary: HRP conjugated Goat Anti-Rabbit IgG(at 1: 3000 dilution;
  Predicted band size : 53kD
  Observed band size : 53kD
  

  

  Fig3: Sample: Placenta (Mouse) Lysate at 30 ug
  Primary: Anti- CYP11A1 (Ser576) (ER1906-98) at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
  Predicted band size: 53/57 kD
  Observed band size: 57 kD

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