种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit
验证应用WB
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human Cyclin F 451-550/786
偶联Non-conjugated
Anti-Cyclin F antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Nucleus. Cytoplasm, cytoskeleton, centrosome, centriole. Note=Localization in the centrosome is rare in S phase cells and increases in G2 cells, Localizes on both the mother and daughter centrioles. Localization to centrosomes is not dependent on CP110. A
其它名称CCNF
F box only protein 1
FBX1
FBXO1
G2 mitotic specific cyclin F
CCNF_HUMAN.
Anti-Cyclin F antibody应用
WB:1:500-2000
Fig1: Sample:
U-2OS(Human) Cell Lysate at 30 ug
Primary: Anti- Cyclin F at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 86 kD
Observed band size: 86 kD
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