Anti-Cyclin E1 antibody

Anti-Cyclin E1 antibody

• 种属反应性Human,Mouse,Rat

• 验证应用WB,IHC-P,ICC/IF,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from rat Cyclin E 375-411/411

• 偶联Non-conjugated

• Anti-Cyclin E1 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Nucleus.

  
  

• 其它名称CCNE 1
  CCNE
  CCNE1
  Cyclin Es
  Cyclin Et
  CyclinE
  G1/S specific cyclin E
  G1/S-specific cyclin-E1
  CCNE1_HUMAN
  pCCNE1.

  more

• Anti-Cyclin E1 antibody应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:1μg/Test
  IF:1:100-500

• 

  Fig1: Sample:
  Brain(Rat) lysate at30ug;
  Lung(Rat) lysate at 30ug;
  Primary: Anti-Cyclin E  at 1:200;
  Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000;
  Predicted band size : 45kD
  Observed band size : 45kD
  

  

  Fig2: Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-Cyclin-E Polyclonal Antibody, Unconjugated1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
  

  

  Fig3: Cell: NIH/3T3
  Concentration:1:100
  Host/Isotype:Rabbit/IgG
  Flow cytometric analysis of primary antibody  on NIH/3T3(green) compared with Rabbit IgG isotype control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .
  

  

  Fig4: Sample: Hela Lysate at 40 ug
  Primary: Anti-Cyclin E at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
  Predicted band size: 45 kD
  Observed band size: 48 kD
  

  

  Fig5: Sample:
  Brain(Mouse) Lysate at 40 ug
  Testis(Mouse) Lysate at 40 ug
  Primary: Anti-Cyclin Eat 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
  Predicted band size: 45 kD
  Observed band size: 48 kD
  

  

  Fig6: Blank control (blue line): Mouse spleen cells (blue).
  Primary Antibody (green line): Rabbit Anti-Cyclin E1 antibody
  Dilution: 1μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG .
  Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
  Dilution: 1μg /test.
  Protocol
  The cells were fixed with 70% ethanol (overninght at 4℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

  

  Fig7: Sample:
  MCF-7(Human) Cell Lysate at 30 ug
  Primary: Anti-Cyclin E1at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 45 kD
  Observed band size: 48 kD
  

  

  Fig8: Sample:
  Placenta (Mouse) Lysate at 40 ug
  Primary: Anti-Cyclin E1  at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 45 kD
  Observed band size: 48 kD

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！