Anti-CT054 antibody
种属反应性Human,Mouse,Rat,Dog,Cow
验证应用WB,FC
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human CT054/C20orf54 391-469/469
偶联Non-conjugated
Anti-CT054 antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Cell membrane; Multi-pass membrane protein.
其它名称bA371L19.1
BVVLS
C20orf54
C20orf54provided by HGNC
Chromosome 20 open reading frame 54
hRFT2
MGC10698
S52A3_HUMAN
RFT2
Riboflavin transporter 2.
more
Anti-CT054 antibody应用
WB:1:500-2000
FC:3ug/Test
Fig1: Sample:
Testis (Rat) Lysate at 40 ug
Primary: Anti-CT054 at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Fig2: Blank control: Mouse kidney.
Primary Antibody (green line): Rabbit Anti-CT054 antibody
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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