Anti-CPLX1 antibody

Anti-CPLX1 antibody

• 种属反应性Human,Mouse,Rat,Chicken,Pig,Cow

• 验证应用WB,IHC-P,ICC/IF

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human CPLX1 31-100/134

• 偶联Non-conjugated

• Anti-CPLX1 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Cytoplasm; cytosol. Enriched at synaptic-releasing sites in mature neurons.

• 其它名称complexin 1
  Complexin I
  Complexin-1
  CPLX1
  CPLX1_HUMAN
  CPX I
  CPX-I
  CPX1
  Synaphin 2
  Synaphin-2
  921-S.

  more

• Anti-CPLX1 antibody应用

  WB:1:500-2000
  IHC-P:1:400-800
  IF:1:100-500
  ICC/IF:1:100-500

• 

  Fig1: Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CPLX1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CPLX1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

  

  Fig3: Sample:
  RSC96(Rat) Cell Lysate at 30 ug
  Primary: Anti- CPLX1at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 15 kD
  Observed band size: 17 kD

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