Anti-FCGR1A/CD64 antibody

Anti-FCGR1A/CD64 antibody

• 种属反应性Human,Mouse,Rat,Pig,Cow

• 验证应用WB,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human CD64/IGFR1 101-200/374

• 偶联Non-conjugated

• Anti-FCGR1A/CD64 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Cell membrane; Single-pass type I membrane protein. Note=Stabilized at the cell membrane through interaction with FCER1G.

  
  

• 其它名称CD 64
  CD64 antigen
  CD64A
  Fc fragment of IgG high affinity Ia receptor
  F Fc fragment of IgG, high affinity Ia, receptor (CD64)
  Fc fragment of IgG, high affinity Ia, receptor for
  Fc fragment of IgG, high affinity Ib, receptor (CD64)
  Fc fragment of IgG, high affinity Ib, receptor for
  Fc fragment of IgG, high affinity Ic, receptor (CD64)
  Fc fragment of IgG, high affinity Ic, receptor for
  Fc gamma receptor
  Fc gamma receptor I A1
  Fc gamma receptor I
  Fc gamma receptor I B2
  Fc gamma RI
  Fc of IgG high affinity Ia receptor
  Fc-gamma RIC
  FCG 1
  FCG1
  FcgammaRI
  FCGR 1
  FCGR1
  FCGR1A
  FCGR1B
  FCGR1C
  FcRI
  FCRIC
  IGFR 1
  IGFR1
  IGFRB
  IGFRC
  IgG Fc receptor I
  IgG Fc receptor IC
  Immunoglobulin G Fc receptor I
  Immunoglobulin G Fc receptor IB
  Immunoglobulin G Fc receptor IC.

  more

• Anti-FCGR1A/CD64 antibody应用

  WB:1:500-2000
  FC:1μg /test

• 

  Fig1: Blank control(blue): U937(fixed with 2% paraformaldehyde (10 min)).
  Primary Antibody: Rabbit Anti-CD64 antibody, Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
  Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
  

  

  Fig2: Sample:
  U937(Human) Cell Lysate at 30 ug
  Primary: Anti-FCGR1A  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 41 kD
  Observed band size: 41 kD
  

  

  Fig3: Blank control: 293T Cells(blue).
  Primary Antibody: Rabbit Anti-FCGR1A/CD64/AF647 Conjugated antibody  Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
  

  

  Fig4: Blank control: Mouse spleen cells(blue).
  Primary Antibody: Rabbit Anti- FCGR1A/CD64 antibody, Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.
  Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
  Protocol
  Primary antibody  were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

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