Anti-FNDC5 antibody

Anti-FNDC5 antibody

• 种属反应性Human,Mouse,Rat,Chicken,Dog,Rabbit

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human FNDC5 Irisin 81-143/212

• 偶联Non-conjugated

• Anti-FNDC5 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式Affinity purified by Protein A

• 亚细胞定位Cell membrane.

• 其它名称FNDC5
  Fibronectin type III domain containing 5
  Fibronectin type III domain-containing protein 5
  Fibronectin type III repeat containing protein 2
  Fibronectin type III repeat-containing protein 2
  FNDC 5
  Fndc5
  FNDC5_HUMAN
  FRCP2
  Irisin.

  more

• Anti-FNDC5 antibody应用

  WB:1:500-2000
  IHC-P:1:400-800

• 

  Fig1: Sample:
  HepG2 (Human) Cell Lysate at 30 ug
  NIH/3T3 (Mouse) Cell Lysate at 30 ug
  K562 (Human) Lysate at 30 ug
  Heart (Mouse) Lysate at 40 ug
  Primary: Anti-FNDC5  at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 12/20 kD
  Observed band size: 20 kD

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (FNDC5) Polyclonal Antibody, Unconjugated (ER1908-96) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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