Anti-EMP-1 antibody

Anti-EMP-1 antibody

• 种属反应性Human,Mouse,Rat

• 验证应用WB,IHC-P,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from mouse Emp1 101-160/160

• 偶联Non-conjugated

• Anti-EMP-1 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Membrane; Multi-pass membrane protein.

• 其它名称CL-20
  EMP 1
  EMP1
  EMP1_HUMAN
  Epithelial membrane protein 1
  Protein B4B
  TMP
  Tumor-associated membrane protein.

  more

• Anti-EMP-1 antibody应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:3ug/Test

• 

  Fig1: Sample:
  Lung (Mouse) Lysate at 40 ug
  Primary: Anti-EMP-1at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 17 kD
  Observed band size: 19 kD
  

  

  Fig2: Blank control: A431.
  Primary Antibody (green line): Rabbit Anti-EMP-1 antibody 
  Dilution: 3μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG .
  Secondary Antibody: Goat anti-rabbit IgG-AF647
  Dilution: 3μg /test.
  Protocol
  The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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