Anti-Elastin antibody
种属反应性Human,Mouse,Rat
验证应用WB,IHC-P,FC
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human Elastin 681-786/786
偶联Non-conjugated
Anti-Elastin antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Secreted, extracellular space, extracellular matrix. Note=Extracellular matrix of elastic fibers.
其它名称alpha Elastin/Tropoelastin
Elastin isoform a
ELN
ELN_HUMAN
elastin isoform m precursor
FLJ38671
FLJ43523
Supravalvular aortic stenosis
Tropoelastin
Williams Beuren syndrome
Williams syndrome region
ADCL1
SVAS
WBS
WS.
more
Anti-Elastin antibody应用
WB:1:500-2000
IHC-P:1:400-800
FC:1μg /test
Fig1: The figure annotation: The blue histogram is unstained cells.The green histogram is cells stained with Rabbit Anti-alpha Elastin/Tropoelastin antibody plus secondary antibody.
Controls: Positive control: A549 cells .Isotype control: Cell lines treated with rabbit IgG (bs-0295P)instead of the primary antibody to confirm that primary antibody binding is specific.Secondary only control: Both cell lines treated with Goat Anti-rabbit IgG/FITC antibody (bs-0295G-FITC) to confirm no background signal produced from secondary antibody alone. 1ug in 100uL 1 X PBS containing 0.5% BSA.
Fig2: Sample: Lung (Mouse) Lysate at 30 ug
Primary: Anti- alpha Elastin/Tropoelastin at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution
Predicted band size: 70 kD
Observed band size: 68 kD
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