Anti-Apg7 antibody
种属反应性Human,Mouse
验证应用ICC,IHC-P,FC
抗体类型兔多抗
免疫原Peptide
偶联Non-conjugated
Anti-Apg7 antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Peptide affinity purified
亚细胞定位Cytoplasm.
其它名称1810013K23Rik antibody
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Ubiquitin-activating enzyme E1-like protein antibody
Ubiquitin-like modifier-activating enzyme ATG7 antibody
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Anti-Apg7 antibody应用
ICC: 1:500-1:2,000
IHC-P: 1:200-1:1,000
FC: 1:50-1:100
Fig1: ICC staining Apg7 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig2: ICC staining Apg7 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Apg7 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Apg7 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Apg7 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Apg7 antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of Jurkat cells with Apg7 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
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