Anti-GAPDH antibody

Anti-GAPDH antibody

• 种属反应性Human,Mouse,Rat

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型兔多抗

• 免疫原Peptide

• 偶联Non-conjugated

• Anti-GAPDH antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified

• 亚细胞定位Cytoskeleton. Cytosol. Nucleus.

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• 应用

  WB: 1:2,000-5,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1;100

• 

  Fig1: Western blot analysis of GAPDH on different lysates using anti-GAPDH antibody at 1/2,000 dilution.
  Positive control:
  Lane 1: PC-12
  Lane 2: L929
  Lane 3: NIH/3T3
  Lane 4: F9
  Lane 5: Mouse liver tissue
  

  

  Fig2: ICC staining GAPDH in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-GAPDH antibody. Counter stained with hematoxylin.

  

  Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-GAPDH antibody. Counter stained with hematoxylin.

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-GAPDH antibody. Counter stained with hematoxylin.

  

  Fig6: Flow cytometric analysis of MCF-7 cells with GAPDH antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

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