Anti-FOXO3A antibody
种属反应性Human,Mouse
验证应用WB,ICC,IHC-P,FC
抗体类型兔多抗
免疫原Peptide
偶联Non-conjugated
Anti-FOXO3A antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Peptide affinity purified
亚细胞定位Cytoplasm. Nucleus.
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FOX O3A antibody
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foxo3 antibody
FOXO3_HUMAN antibody
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Anti-FOXO3A antibody应用
WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
Fig1: Western blot analysis of FOXO3A on different cell lysate using anti-FOXO3A antibody at 1/500 dilution.
Positive control:
Lane 1: SH-SY5Y
Lane 2: K562
Fig2: ICC staining FOXO3A in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining FOXO3A in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining FOXO3A in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-FOXO3A antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using anti-FOXO3A antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-FOXO3A antibody. Counter stained with hematoxylin.
Fig8: Flow cytometric analysis of SHSY5Y cells with FOXO3A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the seconda
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