Anti-Ki67 antibody

Anti-Ki67 antibody

• 种属反应性Human

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型兔多抗

• 免疫原Synthetic peptide within Human Ki67 aa 1050-1073.

• 偶联Non-conjugated

• Anti-Ki67 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified.

• 亚细胞定位Nucleus.

• 其它名称Antigen identified by monoclonal antibody Ki 67 antibody
  Antigen identified by monoclonal antibody Ki-67 antibody
  Antigen KI-67 antibody
  Antigen KI67 antibody
  Antigen Ki67 antibody
  KI67_HUMAN antibody
  KIA antibody
  Marker of proliferation Ki-67 antibody
  MIB 1 antibody
  MIB antibody
  MKI67 antibody
  PPP1R105 antibody
  Proliferation marker protein Ki-67 antibody
  Proliferation related Ki 67 antigen antibody
  Protein phosphatase 1 regulatory subunit 105 antibody
  RP11-380J17.2 antibody

  more

• Anti-Ki67 antibody应用

  WB: 1:500-1,000
  ICC: 1:200-1:1,000
  IHC-P: 1:100-1:500
  FC: 1:50-1:200

• 

  Fig1: ICC staining Ki67 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig2: ICC staining Ki67 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig3: ICC staining Ki67 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

  

  Fig7: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

  

  Fig8: Flow cytometric analysis of Hela cells with Ki67 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary

  

  Fig9: Western blot analysis of Ki67 on HepG2 cell lysate using anti-Ki67 antibody at 1/1,000 dilution.

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