Anti-Cdc25C antibody
种属反应性Human,Mouse,Rat
验证应用WB,ICC,IHC-P,FC
抗体类型兔多抗
免疫原Peptide
偶联Non-conjugated
Anti-Cdc25C antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Peptide affinity purified.
亚细胞定位Nucleus.
其它名称CDC 25 antibody
Cdc 25C antibody
CDC25 antibody
CDC25C antibody
Cell division cycle 25 homolog C antibody
Cell division cycle 25C antibody
Cell division cycle 25C protein antibody
Dual specificity phosphatase Cdc25C antibody
M phase inducer phosphatase 3 antibody
M-phase inducer phosphatase 3 antibody
Mitosis inducer CDC25 antibody
MPIP3 antibody
MPIP3_HUMAN antibody
Phosphotyrosine phosphatase antibody
PPP1R60 antibody
protein phosphatase 1, regulatory subunit 60 antibody
more
Anti-Cdc25C antibody应用
WB: 1:500-1:1,000
ICC: 1:500-1:2,000
IHC-P: 1:50-1:200
FC: 1:50-1:100
Fig1: Western blot analysis of Cdc25C on human skin tissue lysate using anti-Cdc25C antibody at 1/500 dilution.
Fig2: ICC staining Cdc25C in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Cdc25C in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Cdc25C in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Cdc25C antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-Cdc25C antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Cdc25C antibody. Counter stained with hematoxylin.
Fig8: Flow cytometric analysis of LOVO cells with Cdc25C antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondar
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