Anti-BCL2 antibody

Anti-BCL2 antibody

• 种属反应性Human

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型兔多抗

• 免疫原Peptide.

• 偶联Non-conjugated

• Anti-BCL2 antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified.

• 亚细胞定位Endoplasmic reticulum. Mitochondrion. Nucleus.

• 其它名称Apoptosis regulator Bcl 2 antibody
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  Apoptosis regulator Bcl2 antibody
  AW986256 antibody
  B cell CLL/lymphoma 2 antibody
  B cell leukemia/lymphoma 2 antibody
  Bcl-2 antibody
  Bcl2 antibody
  BCL2_HUMAN antibody
  C430015F12Rik antibody
  D630044D05Rik antibody
  D830018M01Rik antibody
  Leukemia/lymphoma, B-cell, 2 antibody
  Oncogene B-cell leukemia 2 antibody
  PPP1R50 antibody
  Protein phosphatase 1, regulatory subunit 50 antibody

  more

• Anti-BCL2 antibody应用

  WB: 1:500-1:1,000
  ICC: 1:100-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

• 

  Fig1: Western blot analysis of BCL2 on Hela cell lysate using anti-BCL2 antibody at 1/1,000 dilution.

  

  Fig2: ICC staining BCL2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig3: ICC staining BCL2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig4: ICC staining BCL2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-BCL2 antibody. Counter stained with hematoxylin.

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-BCL2 antibody. Counter stained with hematoxylin.

  

  Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-BCL2 antibody. Counter stained with hematoxylin.

  

  Fig8: Flow cytometric analysis of MCF-7 cells with BCL2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary

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