Anti-DKK1 antibody
种属反应性Human,Mouse
验证应用WB,ICC,IHC-P,FC
抗体类型兔多抗
免疫原Peptide
偶联Non-conjugated
Anti-DKK1 antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Peptide affinity purified
亚细胞定位Secreted.
其它名称Dickkopf 1 antibody
Dickkopf 1 homolog antibody
Dickkopf 1 like antibody
Dickkopf homolog 1 antibody
Dickkopf like protein 1 antibody
Dickkopf related protein 1 antibody
Dickkopf WNT signaling pathway inhibitor 1 antibody
Dickkopf-1 antibody
Dickkopf-related protein 1 antibody
DKK 1 antibody
Dkk-1 antibody
Dkk1 antibody
DKK1_HUMAN antibody
hDkk 1 antibody
hDkk-1 antibody
SK antibody
more
Anti-DKK1 antibody应用
WB: 1:500
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
Fig1: Western blot analysis of DKK1 on different tissue lysates using anti-DKK1 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse brain
Lane 2: Human fetal brain
Fig2: ICC staining DKK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining DKK1 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-DKK1 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-DKK1 antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of Hela cells with DKK1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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