Anti-EGFR antibody

Anti-EGFR antibody

• 种属反应性Human,Mouse

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型兔多抗

• 免疫原Peptide.

• 偶联Non-conjugated

• Anti-EGFR antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified

• 亚细胞定位Plasma membrane, Nucleus, Endosome, Endoplasmic reticulum.

• 其它名称Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
  Cell growth inhibiting protein 40 antibody
  Cell proliferation inducing protein 61 antibody
  EGF R antibody
  EGFR antibody
  EGFR_HUMAN antibody
  Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
  Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
  Epidermal growth factor receptor antibody
  erb-b2 receptor tyrosine kinase 1 antibody
  ERBB antibody
  ERBB1 antibody
  Errp antibody
  HER1 antibody
  mENA antibody
  NISBD2 antibody
  Oncogen ERBB antibody
  PIG61 antibody
  Proto-oncogene c-ErbB-1 antibody
  Receptor tyrosine protein kinase ErbB 1 antibody
  Receptor tyrosine-protein kinase ErbB-1 antibody
  SA7 antibody
  Species antigen 7 antibody
  Urogastrone antibody
  v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
  wa2 antibody
  Wa5 antibody

  more

• Anti-EGFR antibody应用

  WB: 1:500-2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

• 

  Fig1: Western blot analysis of EGFR on HepG2 (1) and Hela (2) cell lysates using anti-EGFR antibody at 1/1,000 dilution.

  

  Fig2: ICC staining EGFR in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig3: ICC staining EGFR in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-EGFR antibody. Counter stained with hematoxylin.

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-EGFR antibody. Counter stained with hematoxylin.

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-EGFR antibody. Counter stained with hematoxylin.

  

  Fig7: Flow cytometric analysis of A431 cells with EGFR antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary

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