Fig1: Western blot analysis of ERK2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:2,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Jurkat cell lysate, untreated
Lane 2: A431 cell lysate, untreated
Lane 3: PC-12 cell lysate, untreated
Lane 4: NIH/3T3 cell lysate, untreated
Lane 5: Hela cell lysate, untreated
Lane 6: HT-29 cell lysate, untreated
Lane 7: MCF-7 cell lysate, untreated