Anti-uPA Receptor antibody

Anti-uPA Receptor antibody

• 种属反应性Human,Mouse,Rat,Dog,Cow,Sheep

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human PLAUR 201-300/335

• 偶联Non-conjugated

• Anti-uPA Receptor antibody性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式Affinity purified by Protein A

• 亚细胞定位Isoform 1: Cell membrane; Lipid-anchor, GPI-anchor. Isoform 2: Secreted (Probable).

• 其它名称PLAUR
  Plasminogen activator,urokinase receptor
  Urokinase plasminogen activator surface receptor
  Monocyte activation antigen Mo3
  uPAR
  U-PAR
  MO3
  UPAR
  CD 87
  CD87
  CD87 antigen
  MO 3
  MO3
  Monocyte activation antigen Mo3
  Plasminogen activator urokinase receptor
  U PAR
  u plasminogen activator receptor
  UPA receptor
  UPAR
  Urinary plasminogen activator receptor
  URKR
  Urokinase plasminogen activator receptor
  Urokinase plasminogen activator surface receptor.

  more

• Anti-uPA Receptor antibody应用

  WB:1:500-2000
  IHC-P:1:400-800

• 

  Fig1: Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-uPA Receptor Polyclonal Antibody, Unconjugated 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
  

  

  Fig2: Tissue/cell: mouse adrenal gland; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-uPA Receptor Polyclonal Antibody, Unconjugated1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
  

  

  Fig3: Sample:
  bone (Mouse) Lysate at 40 ug
  Primary: Anti-uPA Receptor at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 31 kD
  Observed band size: 48 kD

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